Corresponding author: Thomas Kaltenbach (
Academic editor: J. Midgley
Material collected between 2017 and 2019 in Ethiopia in the Awash River catchment substantially increased our knowledge of
Kaltenbach T, Gattolliat J-L (2021)
The family
Recently, integrative taxonomy was applied to collections from the highly diverse regions of Southeast Asia and New Guinea, where 65 species were described and named (
Taxonomic studies of
The examined material was collected between 2017 and 2019 during ecological studies of the Awash River (
Sequenced specimens.
Species | Locality | Specimens catalog # | GenBank # ( |
GenSeq Nomenclature |
---|---|---|---|---|
|
Ethiopia | GBIFCH00763723 |
|
genseq-4 |
GBIFCH00763718 |
|
genseq-4 |
||
GBIFCH00763720 |
|
genseq-4 |
||
GBIFCH00763724 |
|
genseq-4 |
||
GBIFCH00763732 |
|
genseq-4 |
||
GBIFCH00763719 |
|
genseq-4 |
||
Saudi Arabia | GBIFCH00517527 |
|
genseq-2 |
|
GBIFCH00235747 |
|
genseq-2 |
||
GBIFCH00235757 |
|
genseq-2 |
||
GBIFCH00517526 |
|
genseq-2 |
||
GBIFCH00465155 |
|
genseq-2 |
||
Ethiopia | GBIFCH00763725 |
|
genseq-2 |
|
GBIFCH00674636 |
|
genseq-2 |
||
|
Ethiopia | GBIFCH00763728 |
|
genseq-4 |
Saudi Arabia | GBIFCH00465151 |
|
genseq-4 |
|
GBIFCH00235741 |
|
genseq-4 |
||
GBIFCH00235750 |
|
genseq-4 |
||
GBIFCH00235731 |
|
genseq-4 |
||
GBIFCH00517523 |
|
genseq-4 |
||
South Africa | GBIFCH00517537 |
|
genseq-4 |
|
GBIFCH00517539 |
|
genseq-4 |
||
GBIFCH00517538 |
|
genseq-4 |
||
Mayotte | GBIFCH00517531 |
|
genseq-4 |
|
GBIFCH00521580 |
|
genseq-4 |
||
GBIFCH00517530 |
|
genseq-4 |
||
|
Ethiopia | GBIFCH00763729 |
|
genseq-4 |
|
Ethiopia | GBIFCH00763731 |
|
genseq-4 |
GBIFCH00763721 |
|
genseq-4 |
||
GBIFCH00763727 |
|
genseq-4 |
||
GBIFCH00674637 |
|
genseq-4 |
||
Saudi Arabia | GBIFCH00517520 |
|
genseq-2 |
|
GBIFCH00517521 |
|
genseq-2 |
||
GBIFCH00235735 |
|
genseq-2 |
||
GBIFCH00235732 |
|
genseq-2 |
||
GBIFCH00465152 |
|
genseq-2 |
||
GBIFCH00465154 |
|
genseq-2 |
Map of Africa with Ethiopia (orange) including the Awash River catchment (green).
So far, the diversity of
All specimens were collected between 2017 and 2019 by Wolfram Graf (University of Natural Resources and Life Sciences, Austria) and Yonas Terefe (Ambo University, Ethiopia) and preserved in 70–96% ethanol.
The dissection of nymphs was performed in Cellosolve (2-Ethoxyethanol) with subsequent mounting on slides with Euparal liquid, using an Olympus SZX7 stereomicroscope.
The DNA of part of the specimens was extracted using non-destructive methods allowing subsequent morphological analysis (see
The GenBank accession numbers are given in Table
Drawings were made using an Olympus BX43 microscope. To facilitate the determination of the new species and the comparison of important structures with other species, we partly used a combination of dorsal and ventral aspects in one drawing (see
Photographs of nymphs were taken using a Canon EOS 6D camera and the Visionary Digital Passport imaging system (
The distribution maps were generated with SimpleMappr (
GPS coordinates of locations of examined specimens.
|
|
|
|
Ethiopia: Mille River |
|
Ethiopia: Korkada |
|
|
Ethiopia: Lafessa |
|
|
Ethiopia: Worer |
|
|
Ethiopia: Awash |
|
|
|
Ethiopia: Borkana River |
|
Ethiopia: Lafessa |
|
|
Ethiopia: Dubti |
|
|
Ethiopia: Worer |
|
|
Ethiopia: Sulula |
|
|
|
Ethipoia: Lafessa |
|
|
Ethiopia: Dubti |
|
Ethiopia: Worer |
|
|
Ethiopia: Wonji |
|
|
Ethiopia: Lafessa |
|
|
Ethiopia: Awash Kunture |
|
|
Ethiopia: Yimre |
|
|
|
Ethiopia: Lafessa |
|
Ethiopia: Korkada |
|
The dichotomous key was elaborated with the support of DKey version 1.3.0 (
The terminology follows
(
Ethiopia • 6 nymphs; Lower Mille River;
The specimens were collected at altitudes between 480 m and 1600 m. Further characteristics of sampling sites are given in
Ethiopia (Fig.
Distribution of
Both mandibles with lateral margins almost straight. Basal half with fine, simple setae scattered over dorsal surface.
Referring to the strongly developed excavation at inner, distolateral margin of maxillary palp segment II.
The specimens were collected at an altitude of 2400 m in relatively cold water (15.9 °C; see
Ethiopia (Fig.
Ethiopia • 6 nymphs; Middle Borkana River;
The specimens were collected at altitudes from 370 m to 1920 m. Further characteristics of sampling sites are given in
Ethiopia (Fig.
Ethiopia • 4 nymphs; Lafessa;
The specimens were collected at an altitude of 1600 m. Further characteristics of the sampling site are given in
Ethiopia (Fig.
Ethiopia • 2 nymphs; Wonji;
The specimens were collected at altitudes from 370 m to 2000 m. Further characteristics of sampling sites are given in
Ethiopia (Fig.
Judging from the figures and description in
Ethiopia • 6 nymphs; Lafessa;
The specimens were collected at altitudes of 1260 m and 1600 m. Further characteristics of sampling sites are given in
Ethiopia (Fig.
1 | Six pairs of gills |
|
– | Seven pairs of gills |
|
2 | With distolateral process at scape |
|
– | Without distolateral process at scape |
|
3 | Maxillary palp with a strongly developed distolateral excavation (Fig. |
|
– | Maxillary palp with distolateral excavation, femur dorsally with a row of 13 to 18 spine-like setae on margin, paraproct with 21 to 29 marginal spines ( |
|
4 | Labial palp segment II with broad thumb-like distomedial protuberance ( |
|
– | Labial palp segment II with narrow thumb-like distomedial protuberance ( |
|
5 | Body dorsally with pattern as in |
|
– | Body dorsally with pattern as in |
|
Intraspecific (bold) and interspecific genetic distances of the sequenced specimens (
Species | Locations | 1 | 2 | 3 | 4 | 5 | |
---|---|---|---|---|---|---|---|
1 |
|
Ethiopia, Saudi Arabia |
|
||||
|
|||||||
2 | Ethiopia | 19 |
|
||||
18–20 | |||||||
3 |
|
Ethiopia, Saudi Arabia, South Africa, Mayotte | 19 | 22 |
|
||
18–20 | 21–23 |
|
|||||
4 |
|
Ethiopia | 19 | 21 | 20 | – | |
19–20 | 21 | 20–21 | |||||
5 |
|
Ethiopia, Saudi Arabia | 18 | 20 | 19 | 18 |
|
17–19 | 19–20 | 18–20 | 17–18 |
|
For the assignment of the new species to
Remarkably, all Afrotropical species of
The distribution of the
Since its description as a new species by
In comparison to
The interspecific genetic distances found in Ethiopia (17–23%, Table
Two species,
The
The number of sampled localities and different habitats in Ethiopia is still limited and there are regions without any collection activities so far (Fig.
We sincerely thank Wolfram Graf (University of Natural Resources and Life Sciences, Austria) and Yonas Terefe (Ambo University, Ethiopia) for the collection of this precious material and for making it available to the Museum of Zoology in Lausanne (